The film lacks explicit evidence of LGBTQ+ characters or narratives. The story focuses on the interaction between Betty Boop, Koko, and the live-action studio environment.

Betty Boop serves as a central figure, providing a baseline for female presence in a leading role. However, her characterization remains tied to the era's specific gender dynamics.

The narrative does not indicate a diverse cast or the use of race-bent casting. It reflects the homogeneous character designs common in 1934 Western animation.

The plot uses surrealism and laughing gas to disrupt reality and traditional stability. This creates a sense of absurdity that departs from rigid, singular moralities.

There is no evidence of characters with visible or invisible disabilities within the provided context.