The film adheres to the heteronormative frameworks typical of 1960s Gothic horror. There is no evidence of same-sex intimacy or non-cisnormative gender identities.

The narrative disrupts traditional hierarchies by centering on female agency. While a male doctor acts as protagonist, the driving forces are female-led witches challenging patriarchal structures.

The cast is ethnically homogeneous, reflecting the historical context of a 1960s Italian production. It does not utilize diverse ethnic representation or race-blind casting.

The film critiques institutional authority by framing religious institutions as architects of past violence. This introduces moral relativism through the witches' quest for vengeance.

Characters with physical impairments, such as the servant Igor, function primarily as Gothic tropes. They serve as atmospheric devices rather than nuanced portrayals of independent identity.