The film lacks explicit queer identities or non-heteronormative characters. While it explores obsessive sexuality and perverse relationships, these are treated as biological anomalies rather than explorations of queer identity.

Alraune serves as a disruptive force who challenges male-dominated scientific authority. However, her agency is often framed through the destructive lens of the femme fatale archetype.

The cast is entirely homogeneous, reflecting the white European context of 1930s German Expressionism. No non-Anglo-Saxon identities are present in the narrative.

The story prioritizes secular scientific inquiry over religious dogma. It deconstructs the traditional family unit by replacing it with a synthetic, laboratory-born lineage.

The protagonist's status as a biological anomaly functions as a metaphor for the 'other.' This serves a horror genre purpose rather than providing a nuanced portrayal of disability.