The film explores gender fluidity by transforming a male protagonist into a female persona. This disrupts Victorian heteronormative binaries, presenting Sister Hyde as a gender-nonconforming entity.

The narrative subverts patriarchal hierarchies by replacing the ineffectual Dr. Jekyll with the dominant, autonomous Sister Hyde. It deconstructs Victorian ideals by shifting power from male science to female hedonism.

Set in Victorian London, the film features a largely homogeneous cast. There is no significant evidence of racial blending or non-Anglo-Saxon representation within this localized setting.

The story critiques Victorian moral absolutism through Sister Hyde’s transgressive, anti-social behavior. It uses scientific experimentation to highlight the corruption of established institutional knowledge and social rigidity.

The protagonist's psychological fragmentation serves as a metaphor for mental health instability. However, these elements often function as horror plot devices rather than nuanced portrayals of agency.