The film lacks prominent LGBTQ+ characters or non-cisnormative identities. Interpersonal dynamics remain strictly within a traditional heteronormative framework.

Dr. Elizabeth Shaw serves as the narrative's primary engine, driving both spiritual and investigative momentum. This subverts typical tropes by centering female intellect and agency over male-driven discovery.

The expedition features a multi-ethnic, international crew that avoids a homogeneous Western cast. Characters are defined by professional roles rather than racial stereotypes, emphasizing a globalized perspective.

The story critiques corporate greed and deconstructs religious authority through Dr. Shaw's convictions. The Engineers serve as a post-colonial metaphor regarding the ethics of discovery and imperialistic motives.

Physical trauma and biological fragility are used primarily to drive horror elements. The film lacks nuanced representation of neurodivergence or the agency of those with permanent impairments.