The film lacks LGBTQ+ characters and does not explore non-heteronormative identities. Interpersonal dynamics focus strictly on species-based conflicts without queer subtext.

Selene serves as a powerful protagonist, driving the combat and tactical decisions of the story. This subverts traditional masculine leadership roles and avoids the damsel in distress trope.

The cast is predominantly white, offering little ethnic variety. The supernatural species used in the film do not serve as proxies for racial or ethnic diversity.

The narrative critiques institutional corruption through the unethical experiments of the Antigen organization. However, it relies on standard mad science tropes rather than systemic social critiques.

There are no prominent depictions of visible or invisible disabilities. Neurodivergence and chronic illness are not used as central character drivers or plot devices.