Naruto: The Lost Story - Mission: Protect the Waterfall Village!

The story follows traditional assumptions where everyone is assumed to be straight or cisgender. There are no depictions of same-sex relationships or non-cisgender identities in this particular storyline.

Female characters play significant roles in combat and intellect, matching their male counterparts. The film avoids showing women in submissive roles, highlighting their strength and independence from traditional gender roles.

The setting uses clan-based identities to represent ethnic diversity. Conflicts involving higher-class ninjas reflect discussions on social stratification and lineage-based hierarchies in the real world.

The plot uses 'holy water' to explore ethical dilemmas and question institutional sanctity. It portrays established village structures as susceptible to corruption.

While the franchise often includes characters with unique physiological traits, this specific mission does not clearly use disability as a main factor driving character's ability to drive the story.