The film relies heavily on heteronormative romantic dynamics, primarily through the volatile relationship between Harley Quinn and the Joker. There is a lack of non-cisnormative identities or narratives that challenge traditional romantic structures.

Female characters are central, possessing high levels of combat proficiency and agency. Harley Quinn drives much of the action, while Amanda Waller provides a depiction of decisive, intellectually dominant female authority.

The ensemble functions as a multi-ethnic collective, featuring characters like Deadshot, Katana, and El Diablo. These identities are treated as integral to their histories rather than mere aesthetic choices.

The narrative critiques Western institutional authority by framing the government agency as an exploitative entity. It also explores unconventional communal bonds through a 'found family' trope among outcasts.

Killer Croc represents physical difference, but the narrative often leans into the 'monster' archetype. This approach uses physical deformity as shorthand for social alienation rather than exploring complex disability.