The film lacks explicit depictions of sexual orientation or gender identity. While the theme of biological 'otherness' offers potential subtext for non-normative identities, no specific characters are confirmed as LGBTQ+.

The ensemble features a balanced distribution of roles, including female characters like Saya and male characters like Subaru. However, the narrative does not explicitly detail the subversion of gender hierarchies.

As a Japanese production, the film depicts a specific ethnic homogeneity. The science fiction focus on mutation serves as a metaphor for biological diversity that transcends traditional racial categories.

The story critiques established power dynamics by portraying state institutions as entities that traumatize the family unit. This deconstructs traditional social structures and the sanctity of the natural family.

The plot centers on individuals whose physical existence is defined by mutations and special abilities. These characters are not mere subjects of study but are the primary drivers of the narrative.