The story lacks explicit non-cisnormative identities or same-sex romantic arcs. The narrative focus remains centered on the protagonist's sibling bond and the group's survival.

The film subverts traditional hierarchies by depicting a patriarchal 'father' figure who uses terror to subjugate female characters. Tanjiro further disrupts tropes by prioritizing empathy over stoic martial dominance.

Set in Taisho-era Japan, the cast is largely homogeneous. The narrative does not use diverse casting to challenge this specific historical setting, focusing instead on internal cultural dynamics.

The arc critiques idealized family units by portraying the spider demon clan as a coercive, predatory hierarchy. It contrasts abusive biological kinship with the empathetic, chosen bonds of the protagonists.

The narrative explores physical combat toll and psychological trauma, but lacks an intentional focus on neurodivergence or visible disabilities. Struggles are primarily framed through supernatural conflict.