The film includes a gay character, moving away from the heteronormative standards typical of survival horror. This inclusion suggests an intentional effort to diversify the character spectrum within a high-stakes thriller.

Casting shows a balanced distribution of male and female leads. However, the film lacks evidence of women subverting traditional roles or exercising agency beyond standard ensemble structures.

A multicultural cast featuring actors like Emílio Dantas and David F. Park disrupts typical Western-centric homogeneity. The Amazon setting provides a natural backdrop for this diverse ensemble.

The narrative focuses on scientific pursuit and biological conflict rather than cultural critique. It leans into individualist survivalist themes common to the found footage genre.

While cancer and disease research are central themes, it is unclear if these provide character agency. There is no evidence of neurodivergent or physically disabled characters driving the plot.