The film lacks explicit LGBTQ+ characters or non-cisnormative identities. The narrative focuses entirely on the biological conflict between Ajin and humans, operating within a traditional character framework.

The story is driven by a male-centric conflict between Kei and Sato. While female characters like Mei exist, they remain on the periphery, serving supporting roles rather than central plot drivers.

Set in contemporary Japan, the cast reflects the local demographic without evidence of whitewashing. However, the film's exploration of 'otherness' is biological rather than ethnic or cultural.

The film offers a sophisticated critique of institutional power and state authority. It portrays government entities as predatory systems, using moral relativism to challenge traditional social orders.

The Ajin condition is treated as a sci-fi phenomenon rather than a nuanced depiction of disability. Medical experimentation serves as a plot device for cruelty rather than exploring neurodivergence or physical disability.