The mysteries focus on traditional investigative tropes. There is no explicit depiction of non-cisnormative identities or same-sex intimacy. Character interactions remain centered on platonic friendship and teamwork.

The ensemble features a balanced gender split that disrupts the lone male hero trope. However, dynamics often lean into gendered archetypes, with female characters frequently occupying roles related to intuition.

The cast is largely homogeneous, reflecting the animation standards of its era. The narrative does not prioritize racial or ethnic intersectionality within the Mystery, Inc. gang.

The stories reinforce traditional Western structures of authority and morality. The protagonists act as amateur vigilantes who uphold social order by unmasking criminals within a binary moral framework.

There is no evidence of characters with visible or invisible disabilities. The investigators are portrayed as able-bodied, with no indication of neurodivergent representation.